https://ogma.newcastle.edu.au/vital/access/ /manager/Index en-au 5 https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:44997 trans-acting small-interfering RNA (tasiRNA) production allowed for use of the loss-of-function mutant lines, drb1, drb2, drb4, and ago7, to further functionally characterize the TAS3 pathway in Arabidopsis thaliana (Arabidopsis). Towards achieving this goal, we also describe the developmental and molecular phenotypes expressed by three newly generated Arabidopsis lines, the drb1ago7, drb2ago7, and drb4ago7 double mutants. We show that the previously reported developmental abnormalities displayed by the drb1, drb2, drb4, and ago7 single mutants, are further exacerbated in the drb1ago7, drb2ago7, and drb4ago7 double mutants, with rosette area, silique length, and seed set all impaired to a greater degree in the double mutants. Molecular assessment of the TAS3 pathway in the floral tissues of the seven analyzed mutants revealed that DRB1 is the sole DRB required for miR390 sRNA production. However, DRB2 and DRB4 appear to play secondary roles at this stage of the TAS3 pathway to ensure that miR390 sRNA levels are tightly maintained. We further show that the expression of the TAS3-derived tasiARF target genes, AUXIN RESPONSE FACTOR2 (ARF2), ARF3, and ARF4, was altered in drb1ago7, drb2ago7, and drb4ago7 flowers. Altered ARF2, ARF3, and ARF4 expression was in turn demonstrated to lead to changes in the level of expression of KAN1, KAN3, and KAN4, three KANADI transcription factor genes known to be transcriptionally regulated by ARF2, ARF3, and ARF4. Taken together, the demonstrated relationship between altered ARF and KAN gene expression in drb1ago7, drb2ago7 and drb4ago7 flowers, could, in part, explain the more severe developmental defects displayed by the double mutants, compared to milder impact that loss of only a single piece of TAS3 pathway protein machinery was demonstrated to have on drb1, drb2, drb4 and ago7 reproductive development.]]> Wed 26 Oct 2022 09:28:43 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:43513 1) and carcinogenic risks (CR > 1.0 × 10−4 ) were greatly affected by different water regimes. The rice plant grown under alternate wetting and drying condition (T7 ) showed the lowest health risks compared to other water management practices. Thus, alternate wetting and drying conditions are a good water management strategy for increasing rice output while reducing arsenic buildup in rice grain.]]> Wed 21 Sep 2022 11:18:26 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:52915 Wed 21 Feb 2024 15:41:34 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:49888 Wed 14 Jun 2023 17:43:48 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:23079 Wed 11 Apr 2018 09:21:28 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:45305 Thu 27 Oct 2022 10:29:16 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:42559 Thu 25 Aug 2022 11:05:11 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:33146 Poaceae family of grasses, namely; maize (Zea mays L.), wheat (Triticum aestivum L.) and rice (Oryza sativa L.). It is, therefore, of significant concern that all three of these Poaceae grass species are susceptible to a range of abiotic stresses, including drought and salt stress. Highly conserved among monocotyledonous and dicotyledonous plant species, microRNAs (miRNAs) are now well-established master regulators of gene expression, influencing all aspects of plant development, mediating defense responses against pathogens and adaptation to environmental stress. Here we investigate the variation in the abundance profiles of six known abiotic stress-responsive miRNAs, following exposure to salt and drought stress across these three key Poaceae grass crop species as well as to compare these profiles to those obtained from the well-established genetic model plant species, Arabidopsis thaliana (L.) Heynh. Additionally, we outline the variables that are the most likely primary contributors to instances of differential miRNA abundance across the assessed species following drought or salt stress exposure, specifically; (1) identifying variations in the experimental conditions and/or methodology used to assess miRNA abundance, and; (2) the distribution of regulatory transcription factor binding sites within the putative promoter region of a MICRORNA (MIR) gene that encodes the highly conserved, stress-responsive miRNA. We also discuss the emerging role that non-conserved, species-specific miRNAs play in mediating a plant’s response to drought or salt stress.]]> Thu 21 Oct 2021 12:52:38 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:36963 Thu 21 Oct 2021 12:51:34 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:36403 Mon 27 Apr 2020 14:01:04 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:49102 1.2 kg) and quality. Cool storage is a low-cost method for the short-term storage of Gac pollen. However, methods for drying pollen to an inactive state need investigation for a storage protocol, and for improvements in fruit set and fruit physicochemical qualities using hand pollination.]]> Fri 05 May 2023 11:25:02 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:49797 Fri 02 Jun 2023 17:08:29 AEST ]]>